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On the next morning, 0.1 ml of overnight bacterial culture was added in 10 ml of fresh M9 medium containing 50 g ml 1 kanamycin and 100 g ml 1 ampicillin, which was further cultured at 37 C, with shaking at 160 rpm until OD 600 = 0.5

Moreover, glyoxalase II (Glo-2) catalyzes S-glutathionylation using (S)-lactoylglutathione [124]