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Based on a previous study 27 , A549 cell lines were pretreated with MitoQ (4 M, dissolved in DMSO, Glpbio, USA) for 2 h before BLM exposure, and subsequent experiments were performed 24 h post-BLM treatment

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In the C32E8.9 OE strain, GFP was detected throughout the body, while mCherry expression was restricted to the pharynx, confirming successful transgene expression

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