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[DOI] [PubMed] [Google Scholar] Di Chiara G, Imperato A (1988) Drugs abused by humans preferentially increase synaptic dopamine concentrations in the mesolimbic system of freely moving rats

2.10 Immunofluorescence assay Following experimental treatments, IEC-6 cells were fixed with 4% paraformaldehyde for 10 min and rinsed three times with PBS (5 min each wash)

Histopathological examination of testis Haematoxylin and eosin staining (H & E) Examining the testicular tissue of sedentary groups revealed normal seminiferous tubules bordered with a profusion of Sertoli cells, many mitotically active spermatogonia, undamaged germinal layers, and normal Leydig cells in the control group of sedentary rats (Fig

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Computational mass spectrometry In total, 36 separate raw mass spectrometry data files (6 plexes with 6 fractions each) were searched in Proteome Discoverer (PD) v2.5 (Thermo Scientific) using the SEQUEST HT Search algorithm