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mansoni TGR was amplified by PCR (forward primer 5-CTATTTGGCTAGACGTCTGT-3 and reverse primer 5-AATACAGTTTCCTTCCCGTT-3) and cloned into PCRII-TOPO vector (Invitrogen, The resulting construct was linearized using XhoI (for SP6 RNA polymerase transcription) and SacI (for T7 RNA polymerase transcription)

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doi:10.1371/journal.pone.0020676, PMID: 21655192 Steullet P, Neijt HC, Cunod M, Do KQ (2006) Synaptic plasticity impairment and hypofunction of NMDA receptors induced by glutathione deficit: relevance to schizophrenia

Likely, T315I-Bcr-Abl cells develop anti-oxidative pathways and metabolic rewiring via ATF4-mediated transcriptional network for growth fitness

Reduced glutathione (GSH) and glutathione disulfide (GSSG) were quantified by capillary (75-m 50-cm silica) electrophoresis (75 mM boric acid and 25 mM Bis-Tris, pH 8.4, 28C, 18 kV) as described previously (Lavoie et al., 2008)

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