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3-HSD activity was measured mixing 200 L of cytosol with 100 mM sodium pyrophosphate buffer (pH 8.9) and 0, 01% of dehydroepiendrosterone ethanol solution

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Environ Health Perspect 25:5765 Srmo EG, Ciesielski TM, verjordet IB, Lierhagen S, Eggen GS, Berg T, Jenssen BM (2011) Selenium moderates mercury toxicity in free-ranging freshwater fish