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LC-MS/MS analysis was performed on an Acquity UPLC H-Class system (Waters) equipped with an Acquity UPLC BEH C18 1.7 m (2.1 50 mm) column (Waters) and an MS detector (Xevo TQD, Waters)

RMSD is an indicator of protein stability, with lower deviations indicating more stable protein structures

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Phytochemicals block glucose utilization and lipid synthesis to counteract metabolic reprogramming in cancer cells

3.4 Anticancer mechanisms of phytosterols and alkaloids 3.4.1 Interference with cholesterol metabolism and tumor cell membrane structure Phytosterols such as -sitosterol are structurally similar to cholesterol and competitively interfere with cholesterol utilization by tumor cells (78)