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BDNF, brain-derived neurotrophic factor, is the central signaling molecule in neuronal survival, synaptic plasticity, and neurogenesis

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Separated proteins were transferred onto Immobilon-P Transfer membranes (MilliporeSigma #IPVH00010), blocked for an hour using 5% milk in TBST, and stained overnight with targeting primary antibodies in 5% milk TBST