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Antigen retrieval was performed in 10 mM Tris (pH 9) by microwaving for 10 min (1100 W) and slides were then blocked with 7% goat serum (v/v) in 1% BSA (w/v)/PBST for 1 h at room temperature

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Under such environment, a fluorophore emits polarized light for a particular molecule

Our results indicate that IGEPAL CA-630 is certainly not a substitute for sonication

On this basis, A was designed to quantify the efficiency of drug delivery for the treatment of pontine gliomas [132]
