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Due to these proliferative effects and expansion of follicular cells, the addition of GH to alginate-based growth media containing bovine secondary follicles produced higher levels of E2 synthesis and secretion ( Interaction of FSH and LH with their complementary gonadotropin receptors (i.e., FSHR and LHR) induces downstream signaling that is critical for steroidogenesis, proliferation, and differentiation, and both signal through the cyclic adenosine monophosphate/protein kinase A (cAMP/PKA) pathway to enhance production of E2 and P4 (Figure 2)

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