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13,17 Pain can be a reason for sleep loss, and sleep disturbance aggravates pain sensitivity

Inoculation was carried out for 2 h at 37C

Coenzyme Q10 (CoQ10) functions as an antioxidant and aids ATP synthesis, whilst L-carnitine facilitates fatty acid transport for energy metabolism

For protein expression, the transformed cells were grown in MGYH medium (1.34% YNB, 1% glycerol, 4 10 5 % biotin, 0.004% histidine) to an OD 600 = 3.06.0 and then induced in MMH medium (1.34% YNB, 0.5% methanol, 4 10 5 % biotin, 0.004% histidine) at 30 C for an additional 48 h

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