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The biofilm suspensions were measured with flow cytometry using an Attune NxT BVXX flow cytometer (ThermoFisher Scientific, USA) and staining was done with 1 v% of 100 times diluted SYBR Green I (10,000 concentrate in DMSO, Invitrogen, Belgium) solution as described in Waegenaar et al

Here is the daily requirement of vitamin B12, as per age

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