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To selectively modify M pro with GSSG on the more reactive cysteine residues, a similar procedure to that above was used except 5 mM GSSG was used and we lowered the buffer pH to 6.8

The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH 2 O and PBS, and then probed with the primary antibody (HA601200) at 1/1,000 dilution for 1 hour at room temperature

cGPX4 can be translocated into the nucleus [11]

Instead, cerebellar and callosal atrophy and periventricular white matter hyperintensities are more frequent

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Beyond the transcriptional control of gene expression, the enzymatic activity of IDH2 could also be regulated by post-translational modifications