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This review differentiates between low-dose methotrexate used in autoimmune diseases and high-dose methotrexate used in oncology, emphasizing mechanistic differences in hepatotoxicity pathways, integrating MASLD-related risk factors, and summarizing current non-invasive diagnostic strategies

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Research has shown that this stimulation occurs in a pulsatile and time-dependent manner, closely aligning with naturally observed secretion rhythms studied in endocrine models