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Multiplying the serum aminotransferase by the acetaminophen concentration to predict toxicity following overdose

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Cells were cultured in DME/Hams F-12 (3:1) supplemented with 10% FCS, 4 mM glutamine, 0.4 g/ml hydrocortisone, 0.1 nM cholera toxin, 5 g/ml insulin, and 10 ng/ml EGF