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Moreover, HKC decreased the expression of interleukin (IL)-1, IL-2, IL-6, and TNF- by suppressing the inflammatory reaction in the kidneys of rats with DN

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Prolonged anesthesia Six week old mice were induced in an anesthesia chamber with 4% isoflurane in oxygen, followed by maintenance anesthesia via facemask with 1.5% isoflurane

doi: 10.1007/s00726-012-1361-4 38 LiuZXiaYZhangXLiuLTuSZhuWet al
This technique was described in a previous publication [79]

So, doctors keep a close eye on how patients do and adjust treatments as needed