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Nanocarrier uptake in midbrain organoids Control or PD organoids were embedded in 10 l Geltrex (Thermo Fischer Scientific) at the bottom of 24-well plates (1 organoid/well), then culture inserts were placed above them containing the BBB model as described in our previous study [24]

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Homogenates were blindly assayed and the concentration of GSH and GSSG was derived from the calibration curves

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This analytical approach demonstrates broad applicability across both water-soluble and fat-soluble antioxidant compounds (Deadman, 1973)

256 In addition, ethanol could activate kinase CK2 to phosphorylate MAT1 at Ser114, promote its interaction with PIN1 isomerase, inhibit MAT1 localization in mitochondria, and promote ethanol-induced mitochondrial dysfunction and fat accumulation