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Quantitative PCR was performed in technical triplicate using 20 ng cDNA, 1 Power SYBR Green PCR Master Mix (Thermo Fisher Scientific) and 0.5 M forward and reverse primers (Supplementary Table 1), using an ABI 7900HT Real-Time PCR System (Applied Biosystems)

Yap5 is an iron-responsive transcriptional activator that regulates vacuolar iron storage in yeast

doi: 10.1002/adma.202312898 [DOI] [PubMed] [Google Scholar] 121.Hong Y, Boiti A, Vallone D, Foulkes NS

By leveraging this technological means, researchers can more effectively integrate biological system data, thereby achieving deeper breakthroughs in the fields of disease mechanism analysis and drug evaluation

S., Townsend, W

Indeed, several products observed in EgtE studies reported here were also observed in ergothioneine oxidation reaction