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Injection technique with 5mg vials Drawing the dose Reconstituted with 2ml water (2,500mcg/ml): 250mcg = 10 units 500mcg = 20 units Process : Wipe vial top with alcohol Draw air into syringe (same amount as dose) Inject air into vial Invert vial Draw solution slowly Remove needle, check for air bubbles Tap syringe to remove bubbles Injection sites Best locations for subcutaneous injection: Abdomen (2 inches from belly button) Front of thigh Back of upper arm (harder to self-inject) Site-specific injection (optional): Can inject near injury site for localized effect

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Grem1 accelerates nucleus pulposus cell apoptosis and intervertebral disc degeneration by inhibiting TGF--mediated Smad2/3 phosphorylation

doi: 10.1038/s41401-023-01185-5