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The breakdown process of BCAAs is similar in all species, initially forming branched-chain -keto acids (BCKAs) via branched-chain amino acid transferase (BCATs) and transferring nitrogen to nitrogen receptors (the most common nitrogen receptor is -ketoglutaric acid (-KG) to form glutamate)

OD 600 was between 0.40.6

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3 Results 3.1 Selective reactions of the non-reduced TrxR1 to thiol-reactive reagents The structures of the thiol-reactive reagents used in this study are shown in Supplementary Table S1

A549 cells were treated with LY294002 (1 M, PI3K inhibitor), afuresertib (5 M, Akt inhibitor), dabrafenib (20 M, Raf inhibitor), AZD6244 (20 nM, MEK inhibitor), and RBC8 (10 M, Ral GTPase inhibitor) for 48 hours, respectively