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After three washes with IP buffer, the loader-bound beads were incubated with 5 M Sth1 3651097 at 4 C for 30 min and washed three times with IP buffer before bound proteins were eluted with SDS-PAGE loading buffer

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RNAi bacteria growth conditions and treatment RNAi bacteria (HT115) containing the L4440 feeding vector are initially grown on LB tetracycline-ampicillin plates (10 mg/ml tryptone, 5 mg/ml yeast extract, 10 mg/ml NaCl, 15 mg/ml agar, 12.5 g/ml tetracycline, 100 g/ml ampicillin) for 16 h at 37 C

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