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Microarray and RNA-Seq-based gene expression profiling studies showed that miR-10a, miR-10b, miR-34c, miR-100, miR-103, miR-196b, miR-365-2 , and miR- 2478 consistently exist in bovine spermatozoa (96, 105, 107)

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Following this, they were washed with PBS and permeabilized with 0.3% (v/v) Triton X-100 (Sigma-Aldrich) solution for 5 min

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