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Description
Briefly, pellets from the bacterial culture were resuspended in periplasmic extraction buffer (100 mM Tris/Cl pH 8.0, 0.5 M sucrose, 1 mM EDTA), incubated for 30 min at 4 C, and centrifuged repeatedly to remove the spheroplasts

Tablets (Cyanocobalamin): May be suitable for individuals with minor deficiencies or for maintenance therapy after initial levels have been corrected with injections

The authors wish to thank Dr Michael Braden and UM's Molecular Core Computational Facility for assistance with the structural modelling, as well as Drs John Gerdes, Michael Kavanaugh and Charles Thompson for their valued discussion
doi: 10.1111/j.1365-2605.1990.tb01042.x 73 DiemerTHalesDWeidnerW
For instance, patients with inflammatory bowel disease, such as Crohns disease, or those with celiac disease , may have trouble absorbing vitamin B12 due to gut damage

Brain health - The tissues in the brain may be more susceptible to oxidative stress