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Immunoblotting The dishes were washed 2 with ice-cold PBS, lysed in 1 Laemli sample buffer, and subjected to standard SDS-PAGE using separate 4%12% Bis-Tris gels, after which the proteins were overnight transferred at 4C to polyvinylidene fluoride membranes

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EMBO Rep 8:912913 Jensen TS, Baron R, Haanpaa M, Kalso E, Loeser JD, Rice AS, Treede RD (2011) A new definition of neuropathic pain

Search strategies differed significantly between WT and Tg4-2 mice on the second day of acquisition training (chi-square, genotype: Day 2 : p = 0.0395), with Tg4-42 mice showing predominantly a random search strategy (61%)