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There have been many studies of high dose vitamin B6 therapy in children with autism [13], with most showing beneficial effects

[PMC free article: PMC441870] [PubMed: 14401058] 87

Immunostaining To perform the immunofluorescence staining against myelin antigens, the sections were washed three times with PBS containing 0.05% Tween 20, then incubated in blocking solution for 1 h at room temperature

Verstovsek S, Kantarjian H, Cortes J, Ravandi-Kashani F, Borthakur G , Nicaise C, Sams I, Giles F, Tefferi A

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Myer, C