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Confirmation most often relies on nonspecific precipitation, which can give misleading results

Nakanishi H, Onose S, Kithara E, et al

Fasting, but not exercise, increases adipose triglyceride lipase (ATGL) protein and reduces G(0)/G(1) switch gene 2 (G0S2) protein and mRNA content in human adipose tissue

Cell 173 , 634648.e12 (2018)

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If data did not meet normality or homogeneity of variance assumptions, nonparametric tests such as the KruskalWallis test were used
