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The cells were first stained using Fixable Viability Stain 510 (FVS, 1:1000), which was followed by surface staining with anti-APC-CyTM7 CD45 BV510 antibodies (1:1000), anti-FITC-mouse CD3 antibodies (1:50), anti-PerCP-Cy5.5-mouse CD4 antibodies (1:50), and intracellular cytokines were then stained with anti-PE-mouse IFN antibodies (1:50) and anti-APC-mouse IL-4 (1:50) or anti-PE-mouse IL-17A antibodies (1:50)

doi: 10.1177/096032719501400102

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