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For CD4 + T cell differentiation, 48-well culture plates were precoated with 2 g/ml -1 (iT reg differentiation), 5 g/ml -1 (T H 1/ T H 2 differentiation) or 10 g/ml -1 (T H 17 differentiation) anti-mouse CD3 and anti-mouse CD28 antibodies overnight at 4 C
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