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Briefly, pellets from the bacterial culture were resuspended in periplasmic extraction buffer (100 mM Tris/Cl pH 8.0, 0.5 M sucrose, 1 mM EDTA), incubated for 30 min at 4 C, and centrifuged repeatedly to remove the spheroplasts

Glutamine administration in patients undergoing cardiac surgery and the influence on blood glutathione levels

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It is also shown in animal models to bolster biochemical feedback mechanisms along the gut-brain axis [2, 4, 22]