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Site-directed mutagenesis Site-directed mutagenesis of the GST clone was performed using a QuikChange mutagenesis kit (Stratagene) according to the manufacturers protocol

The authors would like to thank Alar Mirka, Richard Rosenbaum, Shaban Demirel, Amy Reiter, and Kathy Dodson from Legacy Health Systems for their roles in running the clinical trial

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All movies were imported into cryoSPARC-3.1.0 54 where they were motion corrected, and the CTF was estimated using patch motion correction (Fourier cropped to 1.06 ) and patch CTF estimation, respectively

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