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Calculations of power for case-control studies have been published (Self and Mauritsen 1988

Additional markers to consider: oxidative stress markers (8-isoprostane, malondialdehyde), inflammatory markers (hsCRP, IL-6), liver function tests

doi: 10.3390/polym14081514, PMID: [DOI] [PMC free article] [PubMed] [Google Scholar] 42.Lin M, Bao C, Chen L, Geng S, Wang H, Xiao Z, et al

200 L tissue homogenate was mixed into a tube pre-added with 800 L of ice-cold methanol/water 80% (vol/vol) followed by vortexing rigorously for 1 min, and then centrifuge at ~ 20,160 g for 15 min in a refrigerated centrifuge

[DOI] [PMC free article] [PubMed] [Google Scholar] 71.Cornire N et al

As 125I-labeled melatonin applied intraarterially appeared almost immediately in the liver and then in the bile, it simply indicates that that the liver is the major sink of circulating melatonin, particularly in portal vein and excreted into the bile ( Fig