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They demonstrated that mutant form of PLC that lost its PIP2 hydrolyzing properties still can activate Ras-MAPK kinase pathway suggesting that this activation is due to Ras binding and GEF domain rather than to PIP2 hydrolysis [5]

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Urine samples and plasma samples were diluted with, respectively, MilliQ and 0.9% sodium chloride to achieve concentrations of the compounds that lay within the range of the calibration curve