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This is to assess for: C282Y homozygosity (high risk of iron overload) compound heterozygosity for C282Y and H63D (very low risk of iron overload) simple heterozygosity for C282Y or H63D (no increased risk of iron overload)

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Jun 2000;14(2):84-7

Such increases in the overall variance of molecular processes are predicted by Medawars germline mutation accumulation theory 1 , which states that because older individuals are less likely to contribute their genetic information to the next generation, there is reduced selection to eliminate deleterious phenotypes that appear late in life 2

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This selective effect manifests as a decrease in Bcl-2 and an elevation of cleaved caspase-3 and PARP (Shi et al., 2021a)