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NKX2-2 based nuclei sorting on frozen human archival pancreas enables the enrichment of islet endocrine populations for single-nucleus RNA sequencing

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99.9/0.1 v/v) over 2 minutes, a linear gradient to 100% mobile-phase B over 7 minutes, and then 3 minutes at 100% mobile-phase B

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Bethyl) in 1% BSA/PBS was added

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The GSH responsiveness of NSBSO and NCBSO were validated by incubating them with GSH (10 mM) for 24 h

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No risk of contamination

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Reyes-Contreras et al

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