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50mg vial with 2.5ml bacteriostatic water: 50mg 2.5ml = 20mg/ml For 1mg dose: 1mg 20mg/ml = 0.05ml = 5 units on U-100 syringe 50mg vial with 2ml bacteriostatic water: 50mg 2ml = 25mg/ml For 1mg dose: 1mg 25mg/ml = 0.04ml = 4 units on U-100 syringe 100mg vial with 5ml bacteriostatic water: 100mg 5ml = 20mg/ml For 2mg dose: 2mg 20mg/ml = 0.1ml = 10 units on U-100 syringe Higher concentrations mean smaller injection volumes but require more precise syringe handling

& Deng, X

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Appl Microbiol Biotechnol 41:684688

The meeF-meeY (FY) mutant strain can be rescued by induction of the expression of FtsH, an ATP-dependent metalloprotease that is known to clear jammed SecYEG translocons (Figure 58, left )