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A product ion trigger filter next performed pseudo-spectral matching, triggering an MS 2 event of the endogenous target peptide and (optionally) the single SIL-synthetic peptide (hipMHC) if n 5 product ions were detected from the defined list (+/- 10 ppm)

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Afterward, cells were treated with oligomeric A 40 (10 nM, see Supplementary File for production and characterization), and biochemical assays were subsequently measured 24 h later

M., Eds