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Use the swirl method: aim the BAC water down the vial wall, then swirl or roll the vial between the palms for 30 to 60 seconds

If you have excess reconstituted peptide you cannot use within 28 days, it is better to accept the loss and reconstitute only the amount you will use next time

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If one or more of the ATSDR CVs are available in the site-specific medium, then select the PHAST recommended CV for screening the contaminant(s) in each media (if only acute CVs are available, retain contaminant for further evaluation)