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Cells were then washed twice with PBS and fixed in 4% PFA (Fisher Scientific 50-980-494) in PBS containing 0.1% Triton X-100 (PBST, Sigma-Aldrich T9284) for 20 min at room temperature at dark

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This class of phytochemicals also exhibits anti-inflammatory activities via regulation of cellular activities in inflammatory cells and modulation of enzymatic activities involved in arachidonic acid metabolism (such as phospholipase A2 and COX) and arginine metabolism (such as NOS), as well as modulating the production of other proinflammatory molecules (Hussain et al

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