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Sex steroids regulate the fish responsiveness to both GH stimulators and inhibitors (46, 47), and it is expected that the responsiveness of fish somatotrophs to hypothalamic factors follows a seasonal pattern associated with the sexual and gonadal maturation typical of each species

The detailed protocol is available at protocols.io (Sachett et al., 2021e) 2.9.3 Glutathione peroxidase activity (GPx) The GPx activity in the samples was determined by mixing the sample (30 g of protein) with a reaction medium containing TFK + EDTA (0.5 M, pH 7.0), NADPH (1.6 mM), GSH (10 mM), GR (2.5 U/mL), and 10 mM azide

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MicroRNAs such as miR-214-3p and miR-15a-3p bind to the 3 untranslated region of GPX4 mRNA and suppress its translation, sensitizing cells to ferroptosis

As a next step the difference of medians for each treatment condition was obtained by subtracting the median of the control group from the median of the matching treated group, followed by calculation of weights: weight= number of explants in one treatment group/n