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One molecule of MA reacts with two molecules of thiobarbituric acid when heated in an acidic solution to form a unique Schiff base compound that gives a pink color, and the color can be measured using a visible (at 532535 nm) or fluorescent (excitation 515 nm, emission 553 nm) spectrophotometers [50,51]

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Future studies providing a better understanding of the molecular control and physiological relevance of minor surfactant lipid components are needed

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