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Treating diabetic rats with the combination of enalapril, menhaden oil or -lipoic acid served as a form of rehabilitation therapy, with nearly all the vascular and nerve deficits to revert to near control values with the lone exception of motor nerve conduction velocity, which was also significantly improved compared to diabetic rats but remained significantly decreased compared to control rats

For immunofluorescence studies of spleen sections, mice were humanely killed, and spleens were excised and fixed overnight in periodate-lysine-paraformaldehyde that was prepared as described 59

Ascorbate, lipoic acid, and cyclophilin, but most commonly GSH, can serve as electron donors for disulfide reduction [179,185,191]

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Statistical analysis Results were shown as mean SD from at least three independent experiments

Further, cystine reduction requires NADPH, which is primarily supplied via the pentose phosphate pathway (PPP) from glucose