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Cell viability was evaluated using a CCK8 assay kit, and the UV absorption (405 nm) was measured using a SpectraMax i3x spectrophotometer

[DOI] [PMC free article] [PubMed] [Google Scholar] 174.Shi P., Diez-Freire C., Jun J.Y., Qi Y., Katovich M.J., Li Q., Sriramula S., Francis J., Sumners C., Raizada M.K

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This allows the zinc atom to form an additional coordination bond with the zinc-binding group of the inhibitor 13,14

2 Methods 2.1 Animal procedures The experiment utilized 10-week-old WKY rats (n = 20) as the control group (Norm group) and 10-week-old SHR (n = 20), sourced from Vital River Laboratory Animal Technology Co., Ltd

ScHyr1 acts as a hydroperoxide sensor, forming a disulfide bond with Yap1, and thereby masking a Yap1 nuclear export signal 59,60