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PLGA is insoluble in aqueous media, but it can dissolve in organic solvents, such as acetone and dimethylformamide (DMF) 109

A significant down-regulation of several other enzymes related to energy metabolism was also confirmed at the protein level in riboflavin deficient cells, such as DLD, SDHA, NDUFS1, and NDUFV2

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We also added low levels of diacetyl (0.12 M) to the Buffer solution to reduce the effect of possible abrupt changes in diacetyl concentration that would greatly impact AWA activity