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doi: 10.1074/jbc.M312846200

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For the validation cohort (2021), a similar protocol than above was used in 96 well plates with the following modifications: Digestion was made with 0.25 g of trypsin only, acidification was performed with formic acid 1% (FA)

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Mitochondria produce more than 90% of the cellular energy through oxidative phosphorylation, which involves the tricarboxylic acid cycle and ETC