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Measurement of 4-HNE For immunostaining of 4-HNE-modified proteins, cochlear sections were rehydrated and incubated in Tris buffer (10 mM Tris, 1 mM EDTA, 0.05% Tween20, pH 9.0) at 90 C for 10 min for antigen retrieval

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IV delivery bypasses the digestive system, placing nutrients directly in the bloodstream at concentrations the gut cannot match

Ideally, the diagnosis is made by identifying uric acid crystals in joint fluid or in a mass of uric acid (tophus)