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A probe size of 2.5 was used as was previously used in assessment of a bacterial glutathione transporter 56

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Size exclusion chromatography, which separates proteins based on their size by passing them through a column filled with beads of a specific size

To measure ROS generation in response to the drugs, cells were incubated in 10 M CM-H 2 DCFDA (Invitrogen) for 30 min in the dark at 26 C, after which the drugs were added
Upon activation, NADPH oxidase transfers electrons from NADPH to oxygen, producing superoxide radicals [46]

Usually, it's suggested to detect prepared sample immediately or aliquot sample to refrigerate in a disposable container