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For the pH-change experiments, 50 M PDIA6 and 4 mM CaCl 2 were mixed in 50 mM HEPES-NaOH at different pH values (pH 6.88.0) and PDIA6 droplets were observed after 10 min using a confocal microscope (LSM 800, Carl Zeiss)
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HA in PCOS exacerbates inflammation and OS by increasing inflammatory markers and ROS levels, which damage pancreatic -cells, impairing insulin secretion and further promoting IR [44,45,46]

After injury, the kidney reprograms to shift its energy reliance, which increases its oxygen consumption, becoming more vulnerable to hypoxia and oxidative injury 16,17
