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While electrophoretic mobility shift assays and mass spectrometry data both suggested a potential role of this mixed disulfide for catalysis 26 , kinetic assays later revealed the irrelevance for the catalytic cycle and thus confirmed that the Grx(SSR) species was an artifact 29

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Biochemical assays The total protein content was determined using the method of Lowry et al

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We propose therapies targeting the GSH antioxidant system in tumors in combination with CR may sensitize cancer cells to treatment (64) and reduce the risk of cancerous effects in CR conditions

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