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However, it should be noted that ascorbate may also act as a prooxidant in vivo
In particular, specific gravity tends to work better in highly diluted samples as it is less influenced by metabolic activity, individual variability, kidney function, age and low-muscle mass individuals
This finding might also explain the decreased LDL susceptibility to oxidation observed in patients with homocystinuria

Studies have shown that cells with reduced NAD+ levels exhibit significantly impaired DNA repair capacity, with 40-60% reductions in repair efficiency when NAD+ levels decrease by 50%
A thorough understanding of these practical considerations in the lab will help ensure successful results with this technique
[DOI] [PubMed] [Google Scholar] 54.Gasteyger C., Suter M., Gaillard R.C., Giusti V
