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TLR4 Mediates the Impairment of Ubiquitin-Proteasome and Autophagy-Lysosome Pathways Induced by Ethanol Treatment in Brain
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The tissue samples were homogenized in PBS (pH 7.4) containing 0.1 mmol/L butylated hydroxytoluene (BHT) (TissueRuptor, Qiagen, United States) and sonicated for 30 s in an ice bath (Ultrasonic bath, Branson, United States)
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[PubMed 2260337] 1605
Human -actin was used as an internal control
