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Parameters Sample 1 Sample 2 Sample 3 Expected Conc. (U/L) 12.6 32.5 65 Observed Conc. (U/L) 13.0 33.8 70.2 Recovery rate (%) 103 104 108

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Here, the authors show that early-life high-fat/high-sugar diet induces sex-specific alterations in adult feeding behavior, hypothalamic transcriptome and blood metabolome, with Bifidobacterium longum and prebiotic FOS+GOS administration restoring these effects via distinct mechanisms, highlighting their therapeutic potential.